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August 01, 2013
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Summary
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Rettung von rekombinanten Arenaviren von geklonten cDNAs, ein Ansatz, so genannte Reverse Genetik, ermöglicht es den Forschern, die Rolle der spezifischen viralen Genprodukte, sowie den Beitrag ihrer unterschiedlichen spezifischen Domänen und Rückstände zu untersuchen, um viele verschiedene Aspekte der Biologie von Arenavirus . Ebenso Techniken reverser Genetik in FDA-zugelassenen Zelllinien (Vero) für die Impfstoffentwicklung bietet neue Möglichkeiten für die Erzeugung und sichere Impfstoffe zur Bekämpfung von humanpathogenen Arenaviren.
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Cite this Article
Cheng, B. Y., Ortiz-Riaño, E., de la Torre, J. C., Martínez-Sobrido, L. Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells. J. Vis. Exp. (78), e50662, doi:10.3791/50662 (2013).
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