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Journal
/
Biology
/
抗体在染色<em> C。线虫</em>使用“冻结开裂”
JoVE Journal
Biology
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JoVE Journal
Biology
Antibody Staining in
C. Elegans
Using "Freeze-Cracking"
Please note that all translations are automatically generated.
Click here for the English version.
抗体在染色<em> C。线虫</em>使用“冻结开裂”
DOI:
10.3791/50664-v
•
13:10 min
•
October 14, 2013
•
Janet S. Duerr
1
Department of Biological Sciences
,
Ohio University
Chapters
00:05
Title
01:13
Preparation of Frozen Nematode Slides
04:17
Preparation of Slides of Individual Nematodes
05:32
Fixation: Light Fix of Methanol-Acetone
06:30
Fixation: Hard Fix Using Formaldehyde or Glutaraldehyde
08:22
Preparation of Slides for Microscopy
10:32
Representative Images
12:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
“不许动开裂,”用于暴露线虫
C.
内组织的方法
线虫
抗体用于蛋白质定位,被证实。
Tags
Antibody Staining
C. Elegans
Freeze-cracking
Cuticle
Nematodes
Mechanical Permeabilization
Fixatives
Sample Recovery
Morphology
Optimization
Chemical Permeabilization
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