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对于电感受的制备快速协议<em>大肠杆菌</em>和<em>霍乱弧菌</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Rapid Protocol for Preparation of Electrocompetent
Escherichia coli
and
Vibrio cholerae
Please note that all translations are automatically generated.
Click here for the English version.
对于电感受的制备快速协议<em>大肠杆菌</em>和<em>霍乱弧菌</em
DOI:
10.3791/50684-v
•
07:10 min
•
October 08, 2013
•
Miguel F. Gonzales
,
Teresa Brooks
,
Stefan U. Pukatzki
,
Daniele Provenzano
3
1
Department of Biological Sciences
,
University of Texas Brownsville
,
2
Department of Medical Microbiology and Immunology
,
University of Alberta
,
3
Department of Biomedical Sciences
,
University of Texas Brownsville
Chapters
00:05
Title
02:07
Preparation of Bacterial Cultures, Tools, and Reagents
03:04
Growth and Preparation of Electrocompetent Bacteria
04:01
Transformation of Electrocompetent Bacteria
04:58
Representative Transformation Results
06:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
电穿孔是用于将DNA导入细菌中称为转化的过程中通常采用的方法。为电感受态细胞的制备传统的协议是耗时且劳动密集型的。本文介绍了一种替代,快速,并为电感受态细胞通过一些实验室目前使用的高效制备方法。
Tags
Electroporation
Electrotransformation
Electrocompetent
E. Coli
Vibrio Cholerae
Competent Bacteria
Gene Pulse Controller
Cuvettes
Rapid Protocol
Transformation Method
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