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Micron-skala Opløsning Optical Tomography af hele Mouse Brains med konfokal Light Sheet Microscopy
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
DOI:

09:49 min

October 08, 2013

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Chapters

  • 00:05Title
  • 01:22Dehydration and Clearing
  • 02:37Specimen Mounting
  • 03:33Tomography Preparation
  • 04:26Tomography Acquisition
  • 05:37Stitching and Visualization
  • 07:35Results: Micron-scale Resolution Optical Tomography
  • 08:54Conclusion

Summary

Automatic Translation

I denne artikel beskriver vi den fulde eksperimentelle procedure at rekonstruere, med høj opløsning, den fine hjerne anatomi fluorescensmærkede mus hjerner. Den beskrevne protokol omfatter forberedelse og clearing prøve, prøve montering for billedbehandling, data post-behandling og multi-skala visualisering.

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