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Micron-skala Opløsning Optical Tomography af hele Mouse Brains med konfokal Light Sheet Microscopy
This content is Free Access.
1European Laboratory for Non-linear Spectroscopy (LENS), 2Integrated Research Centre,University Campus Bio-medico of Rome, 3DAEMI,University of Cassino, 4National Institute of Optics (CNR-INO), 5Allen Institute for Brain Science, 6Department of Physics,University of Florence, 7ICON Foundation, Sesto Fiorentino, Italy
Chapters
- 00:05Title
- 01:22Dehydration and Clearing
- 02:37Specimen Mounting
- 03:33Tomography Preparation
- 04:26Tomography Acquisition
- 05:37Stitching and Visualization
- 07:35Results: Micron-scale Resolution Optical Tomography
- 08:54Conclusion
I denne artikel beskriver vi den fulde eksperimentelle procedure at rekonstruere, med høj opløsning, den fine hjerne anatomi fluorescensmærkede mus hjerner. Den beskrevne protokol omfatter forberedelse og clearing prøve, prøve montering for billedbehandling, data post-behandling og multi-skala visualisering.
