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Chapters
- 00:00Title
- 00:50The Components
- 01:47Preparing the Pipette
- 06:17Results: A Real Experiment
- 09:27Conclusion
Vi beskriver her, hvordan du udfører en enkelt-celle mikroinjektion af Lucifer Yellow at visualisere cellulær kommunikation via gap-junctions i levende celler, og give nogle nyttige tips. Vi forventer, at dette dokument vil hjælpe alle til at vurdere graden af cellulære kobling skyldes funktionelle gap junctions. Alt her beskrevne kunne være principielt tilpasses til andre fluorescerende farvestoffer med molekylvægt under 1.000 Dalton.
Tags
Single-cell MicroinjectionCell Communication AnalysisP2 ReceptorsGap JunctionsImmune SystemLiverMicroinjection SetupFluorescence MicroscopeCCD CameraCurrent GeneratorMicroelectrodeMicro-manipulatorBorosilicate Glass CapillaryPolarTungsten FilamentDye LoadingSodium SolutionSilver WireHead StagePetri DishMagnification LensCell TargetingMicroelectrode Testing
