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Chapters
- 00:05Title
- 01:34In vivo Reprogramming and Isolation of Liver Tissue
- 03:40RNA and Flow Cytometry Analysis of Enhanced Pluripotency
- 05:34Immunostaining of Liver Sections for Markers of Pluripotency
- 06:57Alkaline Phosphatase Staining of Liver Tissue Sections
- 07:47Assessment of Liver Toxicity
- 09:00Results: Reprogramming of Somatic Cells Following Hydrodynamic Tail-vein Injection of OKSM Factors
- 11:24Conclusion
This study demonstrates the reprogramming of somatic cells towards pluripotency in vivo without the generation of teratomas. We used hydrodynamic tail vein injection of plasmid DNA encoding the Yamanka factors to induce the in vivo reprogramming of adult hepatocytes into cells of enhanced pluripotency.
