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Medicine
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生产用锌指核酸载脂蛋白C-III淘汰赛兔
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
Please note that all translations are automatically generated.
Click here for the English version.
生产用锌指核酸载脂蛋白C-III淘汰赛兔
DOI:
10.3791/50957-v
•
10:59 min
•
November 18, 2013
•
Dongshan Yang
,
Jifeng Zhang
,
Jie Xu
,
Tianqing Zhu
,
Yanbo Fan
,
Jianglin Fan
,
Y. Eugene Chen
1
Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine
,
University of Michigan Medical Center
,
2
Department of Molecular Pathology
,
University of Yamanashi
Chapters
00:05
Title
01:53
Rabbit Embryo Collection
03:28
Preparation of ZFN mRNA
04:14
Embryo Microinjection
06:38
Embryo Transfer
08:12
Targeted Mutation of ApoCIII
10:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在基因靶向工具最近发展使得生产淘汰赛（KO）的兔子可能。在目前的工作中，我们使用生成的锌指核酸酶（ZFN）五，载脂蛋白（APO）C-III KO兔。这项工作表明，ZFN是一个以生产KO兔高效的方法。
Tags
Apolipoprotein C-III
ApoCIII
Knockout
Rabbits
Zinc Finger Nucleases
Cardiovascular Diseases
Lipid Metabolism
Atherosclerosis
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