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هندسة الأنسجة: بناء ومتعددة الخلايا 3D سقالة لتسليم الطبقات صفائح خلية
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
DOI:

09:24 min

October 03, 2014

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Chapters

  • 00:05Title
  • 01:41Creation of pNIPAAM-coated Plates
  • 03:01Creation of Cell Sheets
  • 04:13Seeding Endothelial Cells into a Neovascular Permissive Hydrogel
  • 05:58Isolation of Cell Sheets
  • 07:25Results: Images of Layered Cellular Patch
  • 08:46Conclusion

Summary

Automatic Translation

For creation of highly organized structures of complex tissue, one must assemble multiple material and cell types into an integrated composite. This combinatorial design incorporates organ-specific layered cell sheets with two distinct biologically-derived materials containing a strong fibrous matrix base, and endothelial cells for enhancing new vessels formation.

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