Journal
/
/
Sample Drift Correction Following 4D Confocal Time-lapse Imaging
JoVE Journal
Bioengineering
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

16,118 Views

10:04 min

April 12, 2014

DOI:

10:04 min
April 12, 2014

5 Views
, ,

Summary

Automatically generated

Time-lapse microscopy allows the visualization of developmental processes. Growth or drift of samples during image acquisition reduces the ability to accurately follow and measure cell movements during development. We describe the use of open source image processing software to correct for three dimensional sample drift over time.

Read Article