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Sample Drift Correction Following 4D Confocal Time-lapse Imaging
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Bioengineering
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JoVE Journal Bioengineering
Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

DOI:
10.3791/51086-v

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10:04 min

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April 12, 2014

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, ,
1School of Biological Sciences,Monash University, 2Janelia Farm Research Campus,Howard Hughes Medical Institute

Chapters

  • 00:00Title/Introduction
  • 01:57Methodology
  • 05:50Representative Results
  • 09:22Conclusion

Summary

Automatic Translation

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Time-lapse microscopy allows the visualization of developmental processes. Growth or drift of samples during image acquisition reduces the ability to accurately follow and measure cell movements during development. We describe the use of open source image processing software to correct for three dimensional sample drift over time.

Tags

Keywords: 4D ConfocalTime-lapse ImagingSample DriftDrift CorrectionCell TrackingFijiImageJLOCI ToolsPhase Correlation3D Registration

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