Sample Drift Correction Following 4D Confocal Time-lapse Imaging

TimeLapse Microscopy allows a visualization of the processes of development growth, or drift of sample that occurs over this image. Acquisition can result in an inability to follow or track the cell movements, the following protocol. We use open source imaging processing software to correct for three-dimensional drift over time.

Capturing a series of optical sections at different focal planes allows the generation of a three dimensional model of the sample. This can be extended into four dimensions by creating a time-lapse series of 3D data sets. In long-term time lapse experiments, it is common to observe sample movement.