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Journal
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Bioengineering
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样品漂移校正继4D共焦时间推移成像
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Sample Drift Correction Following 4D Confocal Time-lapse Imaging
Please note that all translations are automatically generated.
Click here for the English version.
样品漂移校正继4D共焦时间推移成像
DOI:
10.3791/51086-v
•
10:04 min
•
April 12, 2014
•
Adam Parslow
,
Albert Cardona
,
Robert J. Bryson-Richardson
1
School of Biological Sciences
,
Monash University
,
2
Janelia Farm Research Campus
,
Howard Hughes Medical Institute
Chapters
00:00
Title/Introduction
01:57
Methodology
05:50
Representative Results
09:22
Conclusion
Summary
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Automatic Translation
时间推移显微镜可发育过程的可视化。图像采集过程中的增长或样品的漂移减小到准确跟踪和开发过程中测量细胞运动的能力。我们描述了使用开源的图像处理软件,以纠正随着时间的推移三维样品漂移。
Tags
4D Confocal
Time-lapse Imaging
Sample Drift
Drift Correction
Cell Tracking
Fiji
ImageJ
LOCI Tools
Phase Correlation
3D Registration
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