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快速,高效的斑马鱼基因分型采用PCR与高分辨率熔体分析
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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Please note that all translations are automatically generated.
Click here for the English version.
快速,高效的斑马鱼基因分型采用PCR与高分辨率熔体分析
DOI:
10.3791/51138-v
•
06:30 min
•
February 05, 2014
•
Lingyan Xing
2,3
,
Tyler S. Quist
,
Tamara J. Stevenson
,
Timothy J. Dahlem
,
Joshua L. Bonkowsky
2,3,5
1
Division of Pediatric Neurology, Department of Pediatrics
,
University of Utah School of Medicine
,
2
Department of Neurobiology and Anatomy
,
University of Utah School of Medicine
,
3
Interdepartmental Program in Neurosciences
,
University of Utah School of Medicine
,
4
Mutation Generation and Detection Core, HSC Core Research Facility
,
University of Utah School of Medicine
,
5
Department of Neurology
,
University of Utah School of Medicine
Chapters
00:05
Title
01:09
DNA Preparation
02:24
PCR Settings
03:24
PCR Analysis by HRMA
04:31
PCR Identified Mutations
06:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
PCR结合高分辨率的熔融分析(HRMA)被证明是一种快速,有效的方法,以斑马鱼的基因型。
Tags
Zebrafish
Genotyping
PCR
High-resolution Melt Analysis
HRMA
Genetic Tools
Mutation
Transgenic
Screening
Model System
Development
Disease
Drug Screening
Rapid
Efficient
Sensitive
Inexpensive
Contamination
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