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人类神经的聚集体cGMP的适用展开法干细胞和祖细胞来源从多能干细胞或胎儿脑组织
JoVE Journal
Neuroscience
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Neuroscience
A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
Please note that all translations are automatically generated.
Click here for the English version.
人类神经的聚集体cGMP的适用展开法干细胞和祖细胞来源从多能干细胞或胎儿脑组织
DOI:
10.3791/51219-v
•
09:37 min
•
June 15, 2014
•
Brandon C. Shelley
,
Geneviève Gowing
,
Clive N. Svendsen
1
Regenerative Medicine Institute
,
Cedars-Sinai Medical Center
Chapters
00:05
Title
01:36
hNPC Observations
02:13
Chopper Setup
03:22
Pre-chop Procedure
05:58
Chop Procedure
07:08
Post-chop Procedure
08:11
Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
09:04
Conclusion
Summary
Automatic Translation
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这个协议描述了一种新颖的机械斩波方法,它允许球形神经干细胞和祖细胞的聚集体的膨胀而不分离为单个细胞悬浮液。维持细胞/细胞接触可超过40代快速稳定增长。
Tags
CGMP
Expansion Method
Neural Stem Cells
Neural Progenitor Cells
Pluripotent Stem Cells
Neurospheres
Cell Culture
Mechanical Passaging
Automated Chopping
Suspension Culture
Cell Yield
Scale-up
Clinical-grade Cell Production
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