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June 21, 2014
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Hier beschrijven we een protocol gebaseerd op epigenetische herprogrammering van menselijke embryonale stamcellen (hESC 's) in de richting van het genereren van een homogene populatie van de skeletspieren voorlopers die onder permissieve cultuur omstandigheden vormen driedimensionale clusters van samentrekkende spiervezels (myospheres), die biologische kenmerken van de menselijke recapituleren skeletspieren.
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Cite this Article
Albini, S., Puri, P. L. Generation of Myospheres From hESCs by Epigenetic Reprogramming. J. Vis. Exp. (88), e51243, doi:10.3791/51243 (2014).
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