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June 21, 2014
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Summary
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Hier ein Protokoll, das auf epigenetische Reprogrammierung von menschlichen embryonalen Stammzellen (hES-Zellen) in Richtung Erzeugung eines homogenen Bevölkerung von Skelettmuskelvorläuferzellen, die unter permissiven Kulturbedingungen bilden dreidimensionale Cluster von kontraktilen Muskelfasern (myospheres), die biologischen Merkmale der menschlichen rekapitulieren beschreiben wir Skelettmuskeln.
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Cite this Article
Albini, S., Puri, P. L. Generation of Myospheres From hESCs by Epigenetic Reprogramming. J. Vis. Exp. (88), e51243, doi:10.3791/51243 (2014).
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