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Generering av Myospheres Från hESCs genom epigenetiska omprogrammeringen
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Generering av Myospheres Från hESCs genom epigenetiska omprogrammeringen

Article DOI: 10.3791/51243
June 21st, 2014 •
1, 1,2
1Muscle Development and Regeneration Program, Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia

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June 21st, 2014

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Här beskriver vi ett protokoll som bygger på epigenetisk omprogrammering av mänskliga embryonala stamceller (hESCs) mot att generera en homogen population av skelettmuskelgångare som enligt tillåtodlingsbetingelser bildar tredimensionella kluster av kontraktila myofibers (myospheres), som rekapitulera biologiska funktioner i människo skelettmuskulaturen.

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