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Journal / Neuroscience / In vivo Afhøring af centralnervesystemet…
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Neuroscience

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JoVE Journal Neuroscience
In vivo Afhøring af centralnervesystemet Translatome af Polyribosome Fraktionering
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In vivo Afhøring af centralnervesystemet Translatome af Polyribosome Fraktionering

Article DOI: 10.3791/51255-v • 09:13 min • April 30th, 2014
April 30th, 2014 •
1, 1, 1, 1
1Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)

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Denne protokol illustrerer væsentlige modifikationer af polyribosome fraktionering med henblik på at studere translatome in vivo CNS prøver. Det giver mulighed for en samlet vurdering af oversættelse og transkription regulering gennem isolation og sammenligning af total RNA til ribosom bundne RNA fraktioner.

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Neuroscience centralnervesystemet CNS oversættelse polyribosome fraktionering RNA hjerne rygmarv microarray næste generation sekventering gradient translatome
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