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In vivo Afhøring af centralnervesystemet Translatome af Polyribosome Fraktionering
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

In vivo Afhøring af centralnervesystemet Translatome af Polyribosome Fraktionering

DOI:
10.3791/51255-v

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09:13 min

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April 30, 2014

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, , ,
1Department of Molecular Neurobiology,German Cancer Research Center (DKFZ)

Chapters

  • 00:05Title
  • 01:45Spinal Cord and Brain Tissue Preparation
  • 03:28Myelin Flotation
  • 04:33Sucrose Gradient Fractionation
  • 06:23RNA Isolation from Individual Fractions
  • 07:36RNA Profiles from Spinal Cord and Brain Tissue
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol illustrerer væsentlige modifikationer af polyribosome fraktionering med henblik på at studere translatome in vivo CNS prøver. Det giver mulighed for en samlet vurdering af oversættelse og transkription regulering gennem isolation og sammenligning af total RNA til ribosom bundne RNA fraktioner.

Tags

Polyribosome FractionationTranslatomeGene ExpressionMRNA TranslationCentral Nervous SystemNeurobiologyTranscriptomicsProteomicsCycloheximideMyelinSucrose Gradient

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