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JoVE Journal Biology
민감한, 대규모 양적 대사 체학을위한 전략
Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics
Journal (Biology)
Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Journal (Biology)
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
 
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민감한, 대규모 양적 대사 체학을위한 전략

Article DOI: 10.3791/51358
May 27th, 2014 •
1, 1, 1,2, 1,2, 1,2
1Division of Nutritional Sciences, Cornell University, 2Field of Biochemistry, and Molecular Cell Biology, Cornell University

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May 27th, 2014

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대사 산물 프로파일 링은 건강과 질병에있는 신진 대사의 연구에 소중한 자산이다. 극성 스위칭 및 급속한 듀티 사이클 고분해능 질량 분석법에 결합 통상 대별 액체 크로마토 그래피를 이용해서, 우리는 높은 감도, 정밀도 및 해상도를 가진 생물학적 물질의 극성 대사 조성을 분석하는 프로토콜을 기술한다.

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