Journal
/
/
मात्रात्मक RT-पीसीआर से व्यक्ति मानव भ्रूण स्टेम कोशिकाओं की रूपरेखा
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
DOI:

09:03 min

May 29, 2014

, ,

Chapters

  • 00:05Title
  • 01:20Preparing hESCs for FACS Purification
  • 02:26Lysis of FACS-purified Single Cell in Each Well of a 96-well Plate
  • 03:28Reverse Transcription
  • 04:29Amplification
  • 05:26qRT-PCR Performance
  • 06:21Results: qRT-PCR is an Efficient Method for Profiling Single Cell Gene Expression
  • 08:39Conclusion

Summary

Automatic Translation

सिंगल सेल जीन अभिव्यक्ति परख स्टेम सेल विषमताओं को समझने के लिए आवश्यक है.

Related Videos

Read Article