JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Profiling Individuelle humanen embryonalen Stammzellen durch quantitative RT-PCR
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Please note that all translations are automatically generated.
Click here for the English version.
Profiling Individuelle humanen embryonalen Stammzellen durch quantitative RT-PCR
DOI:
10.3791/51408-v
•
09:03 min
•
May 29, 2014
•
HoTae Lim
,
In Young Choi
,
Gabsang Lee
1
Institute for Cell Engineering, Department of Neurology and Neuroscience
,
Johns Hopkins University School of Medicine
Chapters
00:05
Title
01:20
Preparing hESCs for FACS Purification
02:26
Lysis of FACS-purified Single Cell in Each Well of a 96-well Plate
03:28
Reverse Transcription
04:29
Amplification
05:26
qRT-PCR Performance
06:21
Results: qRT-PCR is an Efficient Method for Profiling Single Cell Gene Expression
08:39
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
Einzelzell-Genexpression Test wird für das Verständnis der Stammzell Heterogenitäten benötigt.
Tags
Single Cell
Transcriptome
QRT-PCR
Heterogeneity
Stem Cell
Differentiation
OCT4
NANOG
Gene Expression
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Vermehrung von humanen embryonalen Stammzellen (ES-Zellen)
Ableitung von hämatopoetischen Stammzellen aus embryonalen Stammzellen der Maus
Gewinnung menschlicher embryonaler Stammzellen durch Immunosurgery
Probing für Mitochondriale komplexe Aktivität in humanen embryonalen Stammzellen
Chromatin Immunopräzipitation von humanen embryonalen Stammzellen
In-vitro-Markierung von humanen embryonalen Stammzellen für Magnetic Resonance Imaging
Kultur und Wartung von humanen embryonalen Stammzellen
Chromosomale Spread-Vorbereitung von humanen embryonalen Stammzellen für die Karyotypisierung
Einfrieren und Auftauen humanen embryonalen Stammzellen
Differenzierung von embryonalen Stammzellen in Oligodendrozyten Precursors
Read Article