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Chapters
- 00:05Title
- 01:19Protein Purification and Alkylation of Free Sulfhydryl Groups
- 02:59Protein Digestion, Reversed Phase Peptide Extraction, and Reductive Dimethylation Labeling
- 06:02Basic pH Reversed Phase Chromatography and STop and Go Extraction
- 08:17Microcapillary LC-MS/MS and Peptide Quantification
- 09:58Results: Accuracy, Precision, and Reproducibility of ReDi Labeling
- 11:24Conclusion
Tags
Quantitative ProteomicsReductive DimethylationStable Isotope LabelingMass SpectrometryProtein Expression DifferencesRegular/formaldehyde LabelingDeuterated LabelingSodium CyanoborohydrideMethyl TagsLC-MS/MS AnalysisIon Chromatogram Peak AreasSample PreparationReversed-phase Solid Phase ExtractionBasic PH Reversed-phase ChromatographyStageTip Peptide PurificationAdvantages Of ReDi LabelingLimitations Of ReDi LabelingNovel Applications Of ReDi Labeling
