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Neuroscience
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内吞体动力学在生活神经末梢与四维荧光成像可视化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Please note that all translations are automatically generated.
Click here for the English version.
内吞体动力学在生活神经末梢与四维荧光成像可视化
DOI:
10.3791/51477-v
•
10:51 min
•
April 16, 2014
•
Richard S. Stewart
,
Ilona M. Kiss
,
Robert S. Wilkinson
1
Department of Cell Biology and Physiology
,
Washington University School of Medicine
Chapters
00:05
Title
01:10
Staining the Preparation with Supravital Dyes
02:30
Configuring the Preparation for Imaging
03:31
Setting Parameters for 4D Imaging
04:52
Data Analysis
06:54
Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
10:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
四维(4D)成像被用于研究中两种类型的核内体的活的脊椎动物神经终端的行为和交互作用。这些小的结构的运动的特征是在三维空间中,从而允许确认如内涵体融合和胞吐作用的事件。
Tags
4D Fluorescence Imaging
Endosome Dynamics
Nerve Terminals
Macroendosomes
Acidic Endosomes
3D Time-lapse
Deconvolution
Quantitative Analysis
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