13,088 Views
•
17:08 min
•
August 06, 2014
DOI:
Summary
Automatically generated
Kombinato 5 fluorescerande proteiner märkning av hematopoetiska stamceller och progenitorceller möjliggör in vivo klon spårning via konfokala och två-foton mikroskopi, som ger inblick i benmärg hematopoetisk arkitektur under regenerering. Denna metod tillåter icke-invasiv öde kartläggning av spektralt Kodade HSPCs-härledda celler i intakta vävnader för långa perioder efter transplantation.
Related Videos
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
Related Videos
23595 Views
08:15
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
Related Videos
9688 Views
14:22
Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
Related Videos
13044 Views
12:03
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Related Videos
18576 Views
12:54
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Related Videos
13775 Views
08:12
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
Related Videos
7256 Views
07:40
Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
Related Videos
12984 Views
07:17
Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
Related Videos
7676 Views
07:57
Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
Related Videos
12003 Views
10:17
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
Related Videos
2504 Views
Read Article
Cite this Article
Malide, D., Métais, J., Dunbar, C. E. In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy. J. Vis. Exp. (90), e51669, doi:10.3791/51669 (2014).
Copy