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Chapters
- 00:05Title
- 02:28Mouse Cell Collection, Purification, and Transduction
- 06:05Mouse Bone Marrow Transplantation
- 07:45Setup for Confocal and Two-photon Microscopy
- 10:12Imaging Sternum Bone Marrow Using Confocal and 2-photon Microscopy
- 12:014D Time-lapse Imaging
- 13:31Reconstruction and Analysis of 3D Volumes and 4D Time Sequences
- 14:33Representative 3D Reconstructions of Sternal Bone Marrow and Other Organs
- 16:17Conclusion
Kombinatoriske 5 fluorescerende proteiner mærkning af hæmatopoietiske stamceller og stamceller muliggør in vivo klonal sporing via konfokal og to-foton mikroskopi, der giver indsigt i knoglemarv hæmatopoietisk arkitektur under regenerering. Denne metode giver non-invasiv skæbne kortlægning af spektralt-kodede HSPCs-afledte celler i intakte væv til meget lang tid efter transplantation.
