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In vivo Klonale Sporing af hæmatopoietisk stamcelletransplantation og progenitorceller Præget af Fem fluorescerende proteiner ved hjælp af konfokal og multiphoton mikroskopi
JoVE Journal
Biology
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JoVE Journal Biology
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
DOI:

17:08 min

August 06, 2014

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Chapters

  • 00:05Title
  • 02:28Mouse Cell Collection, Purification, and Transduction
  • 06:05Mouse Bone Marrow Transplantation
  • 07:45Setup for Confocal and Two-photon Microscopy
  • 10:12Imaging Sternum Bone Marrow Using Confocal and 2-photon Microscopy
  • 12:014D Time-lapse Imaging
  • 13:31Reconstruction and Analysis of 3D Volumes and 4D Time Sequences
  • 14:33Representative 3D Reconstructions of Sternal Bone Marrow and Other Organs
  • 16:17Conclusion

Summary

Automatic Translation

Kombinatoriske 5 fluorescerende proteiner mærkning af hæmatopoietiske stamceller og stamceller muliggør in vivo klonal sporing via konfokal og to-foton mikroskopi, der giver indsigt i knoglemarv hæmatopoietisk arkitektur under regenerering. Denne metode giver non-invasiv skæbne kortlægning af spektralt-kodede HSPCs-afledte celler i intakte væv til meget lang tid efter transplantation.

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