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DNA的亲和纯化的芯片(DAP芯片)方法来确定目标基因的细菌双组分调控系统
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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Please note that all translations are automatically generated.
Click here for the English version.
DNA的亲和纯化的芯片(DAP芯片)方法来确定目标基因的细菌双组分调控系统
DOI:
10.3791/51715-v
•
12:24 min
•
July 21, 2014
•
Lara Rajeev
,
Eric G. Luning
,
Aindrila Mukhopadhyay
1
Physical Biosciences Division
,
Lawrence Berkeley National Laboratory
Chapters
00:05
Title
01:57
Determine Gene Target for Response Regulator Using Electrophoretic Mobility Shift Assay
03:54
Verify Target Enrichment after Genomic DNA-protein Binding
07:19
DNA Labeling and Array Hybridization
10:11
Results: DAP-chip Analysis of Gene Targets and Binding Sites for Response Regulators
11:51
Conclusion
Summary
Automatic Translation
English (Original)
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这个视频文章介绍了一种
体外
基于微阵列方法来确定两个组件系统响应监管机构的靶基因和结合位点。
Tags
DNA-affinity-purified Chip
DAP-chip
Bacterial Two Component Regulatory Systems
Gene Targets
Transcription Factors
ChIP-chip
Response Regulators
In Vitro Microarray
Affinity Purification
Tiling Array
Binding Site Motifs
Desulfovibrio Vulgaris Hildenborough
Sigma54-dependent Response Regulator
Article
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