Massively Parallel Reporter Assays in Cultured Mammalian Cells

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Massively Parallel Reporter Assays in Cultured Mammalian Cells

DOI:

10.3791/51719-v

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11:03 min

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August 17, 2014

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Alexandre Melnikov, Xiaolan Zhang, Peter Rogov, Li Wang, Tarjei S. Mikkelsen

¹Broad Institute

Chapters

00:05Title
01:49Sequence Design and Synthesis
04:17Making the MPRA Library
08:33Transfection, Perturbation, and RNA Isolation
09:23Interpreting MPRA Results
10:16Conclusion

Summary

Automatic Translation

The genetic reporter assay is a well-established and powerful tool for dissecting the relationship between DNA sequences and their gene regulatory activities. Coupling candidate regulatory elements to reporter genes that carry identifying sequence tags enables massive parallelization of these assays.

Massively Parallel Reporter Assays in Cultured Mammalian Cells

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Chapters

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