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Generation og oprensning af human INO80 Chromatin Remodeling komplekser og Subcomplexes
JoVE Journal
Biology
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JoVE Journal Biology
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
DOI:

08:44 min

October 23, 2014

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Chapters

  • 00:05Title
  • 01:23Isolation of Nuclei
  • 02:45Extracting Nuclei with Salt
  • 04:38Immunoaffinity Purification of the Human INO80 or INO80 Subcomplexes
  • 06:53Results: Analysis of the Composition of Immunopurified INO80 Complexes and Subcomplexes
  • 08:01Conclusion

Summary

Automatic Translation

Denne protokol beskriver en fremgangsmåde til generering og oprensning af vildtype og mutant-versioner af humane INO80 kromatin remodellering kompleks. Epitop taggede versioner af INO80 underenheder stabilt udtrykt i HEK293-celler, og komplette komplekser og komplekser mangler specifikke sæt af underenheder oprenses ved immunaffinitetskromatografi.

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