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体外胰腺器官从分散的小鼠胚胎祖细胞
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Biology
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JoVE Journal Biology
In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
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体外胰腺器官从分散的小鼠胚胎祖细胞

DOI:
10.3791/51725-v

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08:35 min

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July 19, 2014

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, , ,
1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences,Swiss Institute for Experimental Cancer Research, 2DanStem,University of Copenhagen

Chapters

  • 00:05Title
  • 01:55Dissection of Dorsal Pancreatic Bud from Mouse Embryos
  • 04:19Plating and Culturing of Dispersed Cells
  • 06:06Results: In Vitro Expansion and Differentiation of Embryonic Pancreatic Progenitors
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

在本协议中所述的三维培养方法概括胰腺发育从胚胎分散小鼠胰腺祖细胞，包括他们的大幅扩张，分化和形态发生成支器官。这种方法适合于成像，利基的功能的干扰和操纵。

Tags

Keywords: In Vitro Pancreas OrganogenesisMouse Embryonic Progenitors3D CulturePancreatic ProgenitorsPancreas DevelopmentPancreatic OrganoidsPancreatic MorphogenesisPancreatic Cell DifferentiationEpithelial PolarizationBranching MorphogenesisMechanical CuesCell Tensegrity

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