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JoVE Journal
Biology

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锌指核酸酶增强基因打靶的人胚胎干细胞
 
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锌指核酸酶增强基因打靶的人胚胎干细胞

Article DOI: 10.3791/51764
August 23rd, 2014

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Summary August 23rd, 2014

Please note that all translations are automatically generated.

Click here for the English version.

记者的细胞系提供了可视化,跟踪和隔离,从不同的人群感兴趣的细胞的方法。然而,基因靶向用常规的同源重组在人胚胎干细胞是非常低效的。在这里,我们将介绍使用锌指核酸酶增强同源重组靶向中枢神经系统中脑特异性转录因子PITX3基因与绿色荧光蛋白。

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