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Biology

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Zink-finger Nuclease Forbedret genmålretning i humane embryonale stamceller
 
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Zink-finger Nuclease Forbedret genmålretning i humane embryonale stamceller

Article DOI: 10.3791/51764
August 23rd, 2014

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Summary August 23rd, 2014

Please note that all translations are automatically generated.

Click here for the English version.

Reporter cellelinjer tilbyder et middel til at visualisere, spore og isolere celler af interesse fra heterogene populationer. Men gen-targeting anvendelse af konventionel homolog rekombination i humane embryonale stamceller er yderst ineffektivt. Heri beskriver vi målrettet CNS midthjernen specifik transkriptionsfaktor PITX3 locus med EGFP hjælp zink-finger nuklease forbedret homolog rekombination.

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