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Fluorescens Imaging med One-nanometer Noggrannhet (FIONA)
JoVE Journal
Biology
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JoVE Journal Biology
Fluorescence Imaging with One-nanometer Accuracy (FIONA)
DOI:

11:56 min

September 26, 2014

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Chapters

  • 00:05Title
  • 01:11TIRM Setup
  • 04:40FIONA on Cy3-DNA
  • 06:28Data Analysis for FIONA on Cy3-DNA
  • 07:29Application of FIONA to Quantify Motor Dynamics at Nanometer Scale
  • 09:10Data Analysis and Finding the Step-size of Myosin Walking
  • 09:59Results: Single Fluorophores Are Localized with Nanometer Precision Using FIONA
  • 11:17Conclusion

Summary

Automatic Translation

Enstaka fluoroforer kan lokaliseras med nanometerprecision med hjälp FIONA. Här en sammanfattning av den FIONA teknik rapporteras, och hur man utför FIONA experiment beskrivs.

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