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Neuroscience
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动物的基因分型快速其次是建立大脑神经元原代培养
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
Please note that all translations are automatically generated.
Click here for the English version.
动物的基因分型快速其次是建立大脑神经元原代培养
DOI:
10.3791/51879-v
•
09:51 min
•
January 29, 2015
•
Jin-Young Koh*
1,2
,
Sadahiro Iwabuchi*
1
,
Zhengmin Huang
,
N. Charles Harata
1
Department of Molecular Physiology & Biophysics
,
University of Iowa Carver College of Medicine
,
2
Department of Psychiatry
,
University of Iowa Carver College of Medicine
,
3
EZ BioResearch LLC
Chapters
00:05
Title
01:33
Fast PCR Genotyping of Newborn Mice
02:56
Primary Culture of Mouse Brain Neurons
08:01
Results: Representative Genotyping of Primary Neuronal Culture
09:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了标签和基因分型新生小鼠,并从中产生的主要神经文化的过程。基因分型是快速,有效和可靠的,并且允许进行自动核酸提取。这是为neonatally致死小鼠和其文化,需要事先完成基因分型的特别有用的。
Tags
Genotyping
Primary Neuronal Culture
Rapid Genotyping
Tattooing
Low-density Culture
DYT1 Dystonia
Hippocampus
Cerebral Cortex
Striatum
Neuronal Morphology
Neuronal Function
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