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Journal
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Neuroscience
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初级量子神经元轴突运输的实时成像点标记的BDNF
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
Please note that all translations are automatically generated.
Click here for the English version.
初级量子神经元轴突运输的实时成像点标记的BDNF
DOI:
10.3791/51899-v
•
10:53 min
•
September 15, 2014
•
Xiaobei Zhao
,
Yue Zhou
,
April M. Weissmiller
,
Matthew L. Pearn
4
,
William C. Mobley
,
Chengbiao Wu
1
Department of Neurosciences
,
University of California, San Diego
,
2
School of Biomedical Engineering and Med-X Research Institute
,
Shanghai Jiao Tong University
,
3
Department of Anesthesiology
,
University of California, San Diego
,
4
VA San Diego Healthcare System
Chapters
00:05
Title
01:32
Preparation of Microfluidic Chambers
03:26
Dissection of Neuronal Culture and Plating on Chambers
05:41
Axonal Transport of Quantum Dot-labeled Brain-derived Neurotrophic Factor (QD-BDNF)
07:43
Results: Live Imaging of Axonal Transport of QD-BDNF in Hippocampal Neurons
10:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
BDNF,神经营养因子,对轴突运输是几个神经元群体的生存和功能是至关重要的。一些退行性疾病的标志是轴索结构和功能的破坏。我们展示了用于检查QD-BDNF在使用初级神经元微流控室现场贩卖的技术。
Tags
Axonal Transport
BDNF
Quantum Dot
Neurodegenerative Disease
Microfluidic Device
Retrograde Transport
Axonal Function
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