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对于耐药性突变对激酶抑制剂的筛选和验证方法
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A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors
Please note that all translations are automatically generated.
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对于耐药性突变对激酶抑制剂的筛选和验证方法
DOI:
10.3791/51984-v
•
12:40 min
•
December 07, 2014
•
Meenu Kesarwani
,
Erika Huber
,
Zachary Kincaid
,
Mohammad Azam
1
Divisions of Experimental Hematology and Cancer Pathology, Cancer Blood Disease Institute
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:05
Title
01:33
Preparation of Random Mutation Library by Random Mutagenisis
03:38
Production of Retroviral Supernatants
05:13
Selection of Resistant Clones
In Vitro
08:15
In Vitro
Validation of Resistant Mutations
09:58
Results: Identification and Validation of Ruxolitinib Resistant Mutations
12:04
Concluion
Summary
Automatic Translation
English (Original)
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Automatic Translation
针对激酶抑制剂治疗遗传性的出现带来了有效的癌症治疗显著的挑战。对新开发的药物鉴定和耐药突变特性有助于更好的临床管理和下一代药物设计。在这里,我们描述了体外筛选及耐药突变的验证协议。
Tags
BCR/ABL
Kinase Inhibitors
Drug Resistance Mutations
Screening Strategy
Retroviral Vector
JAK2
Ruxolitinib
BAF3 Cells
In Vitro Screening
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