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Biology
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从血液高效的iPS细胞生成使用附加体和HDAC抑制剂
JoVE Journal
Biology
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JoVE Journal
Biology
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
Please note that all translations are automatically generated.
Click here for the English version.
从血液高效的iPS细胞生成使用附加体和HDAC抑制剂
DOI:
10.3791/52009-v
•
08:14 min
•
October 28, 2014
•
Jesse J. Hubbard
,
Spencer K. Sullivan
,
Jason A. Mills
,
Brian J. Hayes
,
Beverly J. Torok-Storb
,
Aravind Ramakrishnan
1
Clinical Research Division
,
Fred Hutchinson Cancer Research Center
,
2
Division of Hematology
,
The Children’s Hospital of Philadelphia
,
3
Department of Pathology
,
The Children’s Hospital of Philadelphia
Chapters
00:05
Title
01:23
Ficoll Separation of PBMCs and Expansion of Erythroblasts Days 0-6
03:17
Reprogramming Cells by Nulceofection Day 9
04:51
Plating Feeder Cells Day 10-11
05:47
Plating Cells on Feeder Cells and Changing Medium Day 12
06:41
Results: Representative Nucleofected and Inducible Pluripotent Stem (iPS) Cells
07:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种协议,用于使用基于附加体重编程的策略和组蛋白脱乙酰酶抑制剂的外周血产生的诱导的人多能干细胞。
Tags
IPS Cell Generation
Episomes
HDAC Inhibitors
Peripheral Blood
Integration-free
Nonintegrating Reprogramming Vectors
Erythroblasts
Nucleofection
EBNA1
Mouse Embryonic Fibroblasts
Epigenetic Remodeling
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