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Effektiv iPS Cell Generation fra Blood Brug episomer og HDAC-hæmmere
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JoVE Journal Biology
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Effektiv iPS Cell Generation fra Blood Brug episomer og HDAC-hæmmere

DOI:
10.3791/52009-v

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08:14 min

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October 28, 2014

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, , , , ,
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Division of Hematology,The Children’s Hospital of Philadelphia, 3Department of Pathology,The Children’s Hospital of Philadelphia

Chapters

  • 00:05Title
  • 01:23Ficoll Separation of PBMCs and Expansion of Erythroblasts Days 0-6
  • 03:17Reprogramming Cells by Nulceofection Day 9
  • 04:51Plating Feeder Cells Day 10-11
  • 05:47Plating Cells on Feeder Cells and Changing Medium Day 12
  • 06:41Results: Representative Nucleofected and Inducible Pluripotent Stem (iPS) Cells
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en protokol til generering af menneskeskabte pluripotente stamceller fra perifert blod ved hjælp af et episom baseret omprogrammering strategi og histondeacetylaseinhibitorer.

Tags

IPS Cell GenerationEpisomesHDAC InhibitorsPeripheral BloodIntegration-freeNonintegrating Reprogramming VectorsErythroblastsNucleofectionEBNA1Mouse Embryonic FibroblastsEpigenetic Remodeling

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