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エピソームおよびHDAC阻害剤を用いて血液からの効率的なiPS細胞の生成
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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
Please note that all translations are automatically generated.
Click here for the English version.
エピソームおよびHDAC阻害剤を用いて血液からの効率的なiPS細胞の生成
DOI:
10.3791/52009-v
•
08:14 min
•
October 28, 2014
•
Jesse J. Hubbard
,
Spencer K. Sullivan
,
Jason A. Mills
,
Brian J. Hayes
,
Beverly J. Torok-Storb
,
Aravind Ramakrishnan
1
Clinical Research Division
,
Fred Hutchinson Cancer Research Center
,
2
Division of Hematology
,
The Children’s Hospital of Philadelphia
,
3
Department of Pathology
,
The Children’s Hospital of Philadelphia
Chapters
00:05
Title
01:23
Ficoll Separation of PBMCs and Expansion of Erythroblasts Days 0-6
03:17
Reprogramming Cells by Nulceofection Day 9
04:51
Plating Feeder Cells Day 10-11
05:47
Plating Cells on Feeder Cells and Changing Medium Day 12
06:41
Results: Representative Nucleofected and Inducible Pluripotent Stem (iPS) Cells
07:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、再プログラミング戦略とヒストンデアセチラーゼ阻害剤ベースのエピソームを用いて末梢血からのヒト人工多能性幹細胞を生成するためのプロトコルを記述している。
Tags
IPS Cell Generation
Episomes
HDAC Inhibitors
Peripheral Blood
Integration-free
Nonintegrating Reprogramming Vectors
Erythroblasts
Nucleofection
EBNA1
Mouse Embryonic Fibroblasts
Epigenetic Remodeling
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