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Analysen af ​​neurovaskulære Remodeling i Entorhino-hippocampus organotypisk skivekulturer
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Neuroscience
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JoVE Journal Neuroscience
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures

Analysen af ​​neurovaskulære Remodeling i Entorhino-hippocampus organotypisk skivekulturer

DOI:
10.3791/52023-v

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08:27 min

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October 23, 2014

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, ,
1Anatomical Institute, Department of Biomedicine Basel,University of Basel, 2Department of Neonatology, University Children’s Hospital (UKBB),University of Basel

Chapters

  • 00:05Title
  • 01:20Organotypic Entorhino-hippocampal Slice Cultures
  • 02:37In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
  • 04:28Fixation and Immunostaining
  • 05:47Determination of Regional Vessel Density
  • 06:22Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

En protokol til entorhino-hippocampus organotypisk skivekulturer, som gør det muligt at gengive mange aspekter af iskæmisk hjerneskade, er præsenteret. Ved at studere ændringer i neurovasculature udover ændringer i neuroner denne protokol er et alsidigt værktøj til at studere ændringer plast i nervevæv efter skade.

Tags

Brain IschemiaNeurovascular RemodelingOrganotypic Slice CulturesOxygen And Glucose DeprivationIn Vitro ModelNeuronal DamageVascular Changes

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