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Baseada em imagem Técnica de Citometria de Fluxo para avaliar as mudanças na função de granulócitos<em> In Vitro</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
DOI:

06:13 min

December 26, 2014

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Chapters

  • 00:05Title
  • 01:00Phagocytosis Assay Technique
  • 03:00Sample Analysis
  • 04:43Results: Representative Activated Granulocyte Subsets Images
  • 05:44Conclusion

Summary

Automatic Translation

This method demonstrates a technique for assessing granulocyte function by simultaneously measuring phagocytosis of bacteria and oxidative burst. Image-based flow cytometry allowed for the identification of three distinct subsets of activated granulocytes that all differed in their relative functional capacity.

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