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质粒DNA转座子介导整合到新生小鼠的脑室下区,以生成胶质母细胞瘤的新模型
JoVE Journal
Medicine
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JoVE Journal Medicine
Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
DOI:

10:58 min

February 22, 2015

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Chapters

  • 00:05Title
  • 02:43Preparations for the Intra-ventricular Neonatal Injections
  • 04:15Intra-ventricula Neonatal Injections
  • 06:37Monitoring Plasmid Uptake and Tumor Formation and Progression
  • 08:17Results: Induced Glioblastoma Pathology
  • 09:54Conclusion

Summary

Automatic Translation

Here we describe an efficient and versatile protocol to induce, monitor and analyze novel glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate and generate tumors with histo-pathological characteristics of human GBM.

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