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Chapters
- 00:05Title
- 02:43Preparations for the Intra-ventricular Neonatal Injections
- 04:15Intra-ventricula Neonatal Injections
- 06:37Monitoring Plasmid Uptake and Tumor Formation and Progression
- 08:17Results: Induced Glioblastoma Pathology
- 09:54Conclusion
Here we describe an efficient and versatile protocol to induce, monitor and analyze novel glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate and generate tumors with histo-pathological characteristics of human GBM.
