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文化和共同培养小鼠的卵巢和卵巢卵泡

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

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文化和共同培养小鼠的卵巢和卵巢卵泡

DOI:

10.3791/52458-v

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10:41 min

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March 17, 2015

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Stephanie Morgan, Lisa Campbell, Vivian Allison, Alison Murray, Norah Spears

¹Centre for Integrative Physiology,University of Edinburgh, ²MRC Centre for Reproductive Health,University of Edinburgh

Chapters

00:05Title
01:52Preparation of Dissection and Culture Media
03:44Neonatal Ovary Dissection and Culture
05:32Follicle Dissection, Culture and Co-culture
08:49Results: Environmental Effects on Ovary and Follicle Cell Growth
10:05Conclusion

Summary

Automatic Translation

这个协议描述了小鼠的卵巢组织的原代培养物/共培养，使用卵巢从新生小鼠和从青春期小鼠个体卵泡。文化技术支持发展的高度生理学的方式，允许外在剂对卵巢的影响的调查，和卵泡之间的相互作用。

Tags

Keywords: Mouse Ovary Ovarian Follicles Primordial Follicles Granulosa Cells Oocyte In Vitro Culture Co-culture Follicular Development Ovarian Development

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