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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

DOI:
10.3791/52458-v

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10:41 min

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March 17, 2015

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, , , ,
1Centre for Integrative Physiology,University of Edinburgh, 2MRC Centre for Reproductive Health,University of Edinburgh

Chapters

  • 00:05Title
  • 01:52Preparation of Dissection and Culture Media
  • 03:44Neonatal Ovary Dissection and Culture
  • 05:32Follicle Dissection, Culture and Co-culture
  • 08:49Results: Environmental Effects on Ovary and Follicle Cell Growth
  • 10:05Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.

Tags

Keywords: Mouse OvaryOvarian FolliclesPrimordial FolliclesGranulosa CellsOocyteIn Vitro CultureCo-cultureFollicular DevelopmentOvarian Development

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