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Une analyse comparative des recombinant expression des protéines dans différents bio-usines: bactéries, cellules d'insectes et Plant Systems
JoVE Journal
Biology
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JoVE Journal Biology
A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
DOI:

09:11 min

March 23, 2015

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Chapters

  • 00:05Title
  • 01:23Nicotiana benthamiana Transient Expression System
  • 02:40Nicotiana tabacum Stable Expresssion System
  • 05:28Recombinant Protein Analysis
  • 07:01Results: Representative hGAD65mut Expression in Plant Platforms
  • 08:32Conclusion

Summary

Automatic Translation

In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.

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