23,404 Views
•
09:11 min
•
March 23, 2015
DOI:
Summary
Automatically generated
In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.
Related Videos
09:41
A Method For Production of Recombinant mCD1d Protein in Insect Cells.
Related Videos
10672 Views
07:47
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Related Videos
52968 Views
10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Related Videos
35610 Views
11:44
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Related Videos
14017 Views
08:04
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Related Videos
12162 Views
13:52
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Related Videos
23277 Views
10:43
siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
Related Videos
11340 Views
11:20
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
Related Videos
53516 Views
09:09
Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti
Related Videos
7999 Views
08:12
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
Related Videos
7255 Views
Read Article
Cite this Article
Gecchele, E., Merlin, M., Brozzetti, A., Falorni, A., Pezzotti, M., Avesani, L. A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems. J. Vis. Exp. (97), e52459, doi:10.3791/52459 (2015).
Copy