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Chapters
- 00:05Title
- 01:29Preparing and Fixing Tissues
- 03:04Freezing and Cryosectioning
- 04:26Tyramide Signal Amplification (TSA) for Tracer Visualization
- 08:14Development of the Neural Circuits Controlling the Reproductive Axis
- 10:06Conclusion
Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.
