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JoVE Journal
Biology

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A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
 
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A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

Article doi: 10.3791/52531
April 20th, 2015

Summary April 20th, 2015

Please note that all translations are automatically generated.

Click here for the English version.

In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.

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