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Immunology and Infection

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JoVE Journal Immunology and Infection
Transformation biolistique d'un fluorescent Tagged Gene dans la opportuniste pathogène fongique
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Transformation biolistique d'un fluorescent Tagged Gene dans la opportuniste pathogène fongique

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Transformation biolistique est une méthode utilisée pour générer une intégration stable de l'ADN dans le génome de l'agent pathogène Cryptococcus neoformans opportunistes par recombinaison homologue. Nous démontrerons transformation biolistique d'une construction, qui a la kinase acétate de codage de gène fusionné au marqueur fluorescent mCherry en C. neoformans.

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Biologie moléculaire Numéro 97 disruption génique, Délivrance biolistique acétate kinase PCR de chevauchement la fluorescence
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