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Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
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Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

DOI:
10.3791/52675-v

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09:48 min

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May 04, 2015

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, , , , , ,
1Department of Neurology and Neurotherapeutics,University of Texas Southwestern Medical Center, 2Department of Neurological Surgery,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:37Repetitive Hypoxic Preconditioning
  • 03:01Evans Blue (EB) Injection
  • 04:222,3,5-Triphenyltetrazolium Chloride (TTC) Staining
  • 08:12Results: RHP Reduces Infarct Volumes of Blood-brain Barrier (BBB) Disruption
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes repetitive hypoxic preconditioning, or brief exposures to systemic hypoxia that reduce infarct volumes and blood-brain barrier disruption following transient middle cerebral artery occlusion in mice. It also details dual quantification of infarct volume and blood-brain barrier disruption after stroke to assess the efficacy of neurovascular protection.

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Keywords: Neurovascular ProtectionRepetitive Hypoxic PreconditioningTransient Middle Cerebral Artery OcclusionInfarct VolumeBlood-brain Barrier DisruptionIschemic StrokeMouse ModelCentral Nervous SystemNeuroprotectionPro-inflammatory Disease

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