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Aislamiento de células mononucleares de la del sistema nervioso central de ratas con EAE
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
DOI:

20:51 min

December 04, 2007

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Chapters

  • 00:00Title
  • 00:10Introduction
  • 01:06Cardiac Perfusion
  • 05:36Removing the Brain
  • 09:11Removing the Spinal Cord
  • 11:18Preparation of a Single-Cell Suspension
  • 14:12Percoll Gradient
  • 17:41Harvest of Mononuclear Cells
  • 19:56Conclusion

Summary

Automatic Translation

En este video se demuestra la forma de aislar las células mononucleares del sistema nervioso central de ratones con encefalomielitis autoinmune experimental.

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